Distinguished Professor Antoine van Oijen from the School of Chemistry and Molecular Bioscience, Molecular Horizons, at the University of Wollongong will be giving a public talk Thursday May 30th.
Summary of talk:
Advances in optical imaging and molecular manipulation techniques have made it possible to observe individual enzymes and record molecular movies that provide new insight into their dynamics and reaction mechanisms. In a biological context, most of these enzymes function in concert with other enzymes in multi-protein complexes, so an important direction is the utilization of single-molecule techniques to unravel the orchestration of large macromolecular assemblies. We are applying a single-molecule approach to study DNA replication, a process that is supported by a large, multi-protein complex containing a number of different activities. I will present recent results of single-molecule studies of replication in bacterial and eukaryotic systems, using approaches that rely on mechanical manipulation of individual DNA molecules and the visualization of the fluorescence of individual, labelled replication proteins. Using these methods, we study the molecular mechanisms underlying the coordination at the replication fork of the various enzymatic activities that support DNA unwinding, priming, and synthesis.
Brief Biography of the Speaker:
Antoine van Oijen led research groups at Harvard Medical School and Groningen University (the Netherlands) before moving to the University of Wollongong in 2015 as an ARC Laureate Fellow. His research revolves around the development and use of single-molecule biophysical tools to study complex biological systems. In particular, he is interested in understanding the molecular principles underlying the process of bacterial DNA replication and repair. Using novel single-molecule fluorescence imaging and nano-manipulation techniques, his work has allowed the direct visualization of the dynamics of individual replication and repair complexes and has led to new insights into bacterial genomic maintenance and pathways leading to antibiotic resistance.
Detailed Schedule for Thursday, 30th May 2019:
- 5.30-6.00 pm REFRESHMENTS, University of Wollongong, Building 28, Room 101
- 6.00-7.00 pm LECTURE by Distinguished Professor Antoine van Oijen
- 7.30 pm DINNER with the Speaker at nearby Restaurant
E-mail Dr Fred Osman (email@example.com) to RSVP.